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KMID : 0369820090390010001
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Jorunal of Korean Pharmaceutical Sciences
2009 Volume.39 No. 1 p.1 ~ p.6
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Preparation and Cellular Uptake of Hydrophobic Quantum Dots Encapsulated in Poly-L-Lactic Acid Film
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Lee Ji-Sook
Woo Kyoung-Ja
Chung He-Sson
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Abstract
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To overcome the stability problem of hydrophilic quantum dot (Q-dot), cellular uptake of hydrophobic instead of hydrophilic Q-dot was studied in the hope to find a simple method to use Q-dot as a cellular imaging probe. Hydrophobic Q-dot and poly-L-lactic acid (PLLA) were co-dissolved in chloroform to prepare stable films. Due to the cellular compatibility of PLLA, adherent cells were cultured on the film to observe the degree of Q-dot uptake and cytotoxicity of the prepared films. The results show that Q-dots were absorbed into NIH3T3 and EMT6 cells. Cellular uptake was also observed when hydrophobic Q-dots were coated directly on a glass plate. PLLA/Q-dot film and Q-dot coated on glass plate did not show major cytotoxicity. In vivo tumor model was also used to show the uptake of Q-dot from the PLLA/Q-dot film to the tumor site.
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KEYWORD
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Quantum-dot, PLLA, Cellular Uptake, hydrophobic, tumor imaging
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